Current status and challenges for cell-cultured milk technology: a systematic review

Table 3 Dissociation and sorting methods for the isolated mammary epithelial cells from the mammary gland and milk

Species	Sources	Dissociation enzymes	Sorting methods	Markers	References
Buffalo	Mammary gland	Collagenase, hyaluronidase, and trypsin/EDTA	Selective trypsinization	CK18, vimentin	[45]
Caprine	Mammary gland	Collagenase type 1	Collagen digestion	CK18, CK19, vimentin, α-SMA	[46]
Dairy cow	Mammary gland	Trypsin/EDTA	Selective trypsinization	CK18, vimentin	[47]
Dairy cow	Mammary gland	Trypsin/EDTA	Selective trypsinization	CK18	[48]
Dairy cow	Mammary gland	Trypsin/EDTA	Selective trypsinization	CK18	[49]
Dairy cow	Mammary gland	Trypsin/EDTA	Selective trypsinization	Pan-CK	[50]
Dairy cow	Milk	Not applicable	Centrifuge 1,850 × g, 10 min	Pan-CK	[51]
Dairy cow	Milk	Not applicable	Centrifuge 1,850 × g, 10 min	CK8, Pan-CK	[52, 53]
Goat	Mammary gland	Collagenase type 1, Trypsin/EDTA	Selective trypsinization	CK18	[54]
Human	Mammary gland	Collagenase, hyaluronidase, Accumax	Collagen digestion	CK8, CK14, CK18	[55]
Porcine	Mammary gland	Collagenase A, hyaluronidase	Collagen digestion	CK18, vimentin	[56]
Yak	Mammary gland	Trypsin/EDTA	Selective trypsinization	CK8, CK18, vimentin	[57]

CK Cytokeratin, EDTA Ethylene-diamine-tetraacetic acid, α-SMA α-Smooth muscle actin

ISSN: 2049-1891