Fig. 5
Weighted Gene Co-expression Network Analysis (WGCNA) of annotated lncRNAs in skeletal muscle development. A Scale-free network coefficient (R2) analysis for soft thresholding (β) and corresponding mean connectivity. The red line indicates R2 = 0.85, with β = 5. B Cluster dendrogram based on topological overlap dissimilarity, identifying four modules. C Heatmap showing correlations between module eigengenes and traits associated with growth and development. Each cell includes the correlation coefficient and P value. D Scatter plot of module eigengenes, highlighting the turquoise module, where hub lncRNAs were associated with body weight (module membership = 0.8, gene significance = 0.2). E–F GO (E) and KEGG (F) enrichment analyses of hub lncRNAs