Fig. 4

Effects of a low-protein diet supplemented with rumen-protected amino acids on microbial diversity and structure based on MAGs in the colon of lambs. A Copy numbers of the 16S rRNA genes of total bacteria. B Chao1 index at the MAG level. C Principal coordinate analysis (PCoA) based on Bray–Curtis dissimilarity of microbial composition at the MAG level. D Random forest results for colonic microbiota at species level. ^*P < 0.05