Rumen-protected methionine and lysine supplementation to the low protein diet improves animal growth through modulating colonic microbiome in lambs

Table 3 Effects of a low-protein diet supplemented with rumen-protected amino acids on fermentation characteristics in colon of lambs

Item	Treatments¹			SEM	P-value
Item	CON	LP	LR	SEM	P-value
Ammonia nitrogen, mmol/kg	2.89^b	1.81^c	3.80^a	0.205	< 0.001
Total VFA, mmol/kg	54.50	56.32	54.84	1.698	0.905
VFA molar percentage, mol/100 mol
Acetate	78.83^a	75.24^b	75.24^b	0.610	0.023
Propionate	13.92	14.38	13.84	0.472	0.898
Isobutyrate	0.85^ab	0.57^b	1.07^a	0.071	0.009
Butyrate	4.87^b	8.44^a	7.74^ab	0.535	0.018
Isovalerate	0.61^ab	0.38^b	0.97^a	0.076	0.004
Valerate	1.15^a	0.89^b	1.06^ab	0.071	0.009

¹CON = control diet with crude protein of 112.0 g/kg; LP = low-protein diet with crude protein of 78.4 g/kg; LR = LP + 15 g/d RPMet and 10 g/d RPLys
^a,bValues with a row with different superscripts differ significantly at P < 0.05
VFA Volatile fatty acid

ISSN: 2049-1891